Journal: NPJ Vaccines

Article Title: Immunological correlates of protection following vaccination with glucan particles containing Cryptococcus neoformans chitin deacetylases

doi: 10.1038/s41541-023-00606-0

Figure Lengend Snippet: Wild-type (WT) mice and mice with selected cytokine or cytokine receptor deficiency ( a . IFNγ −/− ; b . IFNγR −/− ; c . IL-6 −/− ; d . IL-23 −/− ; e . TNFα −/− ; f . IL-1β −/− ) received a prime and two biweekly boosts with GP-Cda1 or GP-Cda2. Two weeks after the last boost, mice were challenged with C. neoformans and followed for 70 days for survival. UnVac unvaccinated. n denotes the number of mice in the experimental group. All mice were on the C57BL/6 background. Note the same survival curves are shown for WT mice in Fig. 7a–f. For Fig. 7a–e, P < 0.001 (Mantel–Cox log-rank test) comparing survival between knockout and WT mice vaccinated with either GP-Cda1 or GP-Cda2. For Fig. 7f, P < 0.05 (Mantel–Cox log-rank test) comparing GP-Cda1-vaccinated IL-1β −/− and WT mice.

Article Snippet: Knockout mice on the C57BL/6 background deficient in IFNγ (IFNγ −/− , JAX stock #002287), IFNγR (IFNγR −/− , JAX stock #003288), TNFα (TNFα −/− , JAX stock #005540), IL-1β (IL-1β −/− , JAX stock #068082), IL-6 (IL-6 −/− , JAX stock #002650), and IL-23p19 (IL-23 −/− ) were also obtained from The Jackson Laboratory, except for the IL-23 –/− mice which were from Nico Ghilardi (Genentech, South San Francisco, CA) .

Techniques: Knock-Out

Journal: BMC Developmental Biology

Article Title: Interleukin-6 increases inner cell mass numbers in bovine embryos

doi: 10.1186/s12861-019-0182-z

Figure Lengend Snippet: Transcript abundances for IL6 , IL6R and IL6ST from zygotes, 2-cell embryos, 8-cell embryos, morulae and blastocysts. Total RNA was isolated from 3 to 5 pools of 10 embryos from each developmental stage before reverse transcription. The relative abundance of each target transcript is expressed as fold change from the embryo stage containing the lowest abundance for the specified transcript by using the 2 [-ddCt] approach. Corresponding means and SEMs are indicated by the bars. Different superscripts within each transcript indicates differences ( P < 0.05)

Article Snippet: For all studies, a concentrated IL6 stock (10 μg/ml, recombinant bovine, Kingfisher Biotech, St. Paul, MN, USA) was prepared in SOF containing 1% [ w / v ] bovine serum albumin (BSA; Sigma-Aldrich) and stored in aliquots at − 80 °C.

Techniques: Isolation, Reverse Transcription

Journal: BMC Developmental Biology

Article Title: Interleukin-6 increases inner cell mass numbers in bovine embryos

doi: 10.1186/s12861-019-0182-z

Figure Lengend Snippet: Cleavage and blastocyst formation across each study

Article Snippet: For all studies, a concentrated IL6 stock (10 μg/ml, recombinant bovine, Kingfisher Biotech, St. Paul, MN, USA) was prepared in SOF containing 1% [ w / v ] bovine serum albumin (BSA; Sigma-Aldrich) and stored in aliquots at − 80 °C.

Techniques:

Journal: BMC Developmental Biology

Article Title: Interleukin-6 increases inner cell mass numbers in bovine embryos

doi: 10.1186/s12861-019-0182-z

Figure Lengend Snippet: Embryonic ICM and TE cell counts in Day 8 blastocysts

Article Snippet: For all studies, a concentrated IL6 stock (10 μg/ml, recombinant bovine, Kingfisher Biotech, St. Paul, MN, USA) was prepared in SOF containing 1% [ w / v ] bovine serum albumin (BSA; Sigma-Aldrich) and stored in aliquots at − 80 °C.

Techniques:

Journal: BMC Developmental Biology

Article Title: Interleukin-6 increases inner cell mass numbers in bovine embryos

doi: 10.1186/s12861-019-0182-z

Figure Lengend Snippet: Representative images of differential cell staining in blastocysts collected at day 8 post-fertilization. Embryos either received 0 or 100 ng/ml IL6 beginning at day 5 post-fertilization. Panel A: Blastocysts were harvested at day 8, fixed, immunostained, and physically flattened between a slide and coverslip. Photographs represent a single plane of focus. Nuclei representing TE are indicated by CDX2 + /DAPI + staining (green) and the ICM nuclei are CDX2 − /DAPI + (blue). Control embryo number 1 had 42 ICM cells and 94 TE cells, while control embryo number 2 had 53 ICM cells and 120 TE cells. IL6-treated embryo number 1 had 86 ICM cells and 99 TE cells, while IL6-treated embryo number 2 had 76 ICM cells and 143 TE cells

Article Snippet: For all studies, a concentrated IL6 stock (10 μg/ml, recombinant bovine, Kingfisher Biotech, St. Paul, MN, USA) was prepared in SOF containing 1% [ w / v ] bovine serum albumin (BSA; Sigma-Aldrich) and stored in aliquots at − 80 °C.

Techniques: Staining, Control

Journal: BMC Developmental Biology

Article Title: Interleukin-6 increases inner cell mass numbers in bovine embryos

doi: 10.1186/s12861-019-0182-z

Figure Lengend Snippet: Pooled ICM cell counts and ICM to TE ratios from all studies. All 100 ng/ml treatments of IL6 and associated controls were utilized, regardless of time point of treatment. No other doses of IL6 (1, 10 or 200 ng/ml) are included in this figure. Data from different studies are indicated by different symbols. Panel A : Individual ICM counts for embryos receiving either no treatment or 100 ng/ml IL6. Panel B : Individual ICM:TE ratios for embryos receiving either no treatment or 100 ng/ml IL6. Corresponding means and SEMs are indicated by the bars. Different superscripts within each panel indicates differences ( P < 0.05)

Article Snippet: For all studies, a concentrated IL6 stock (10 μg/ml, recombinant bovine, Kingfisher Biotech, St. Paul, MN, USA) was prepared in SOF containing 1% [ w / v ] bovine serum albumin (BSA; Sigma-Aldrich) and stored in aliquots at − 80 °C.

Techniques:

Journal: BMC Developmental Biology

Article Title: Interleukin-6 increases inner cell mass numbers in bovine embryos

doi: 10.1186/s12861-019-0182-z

Figure Lengend Snippet: Primers used for quantitative RT-PCR

Article Snippet: For all studies, a concentrated IL6 stock (10 μg/ml, recombinant bovine, Kingfisher Biotech, St. Paul, MN, USA) was prepared in SOF containing 1% [ w / v ] bovine serum albumin (BSA; Sigma-Aldrich) and stored in aliquots at − 80 °C.

Techniques: Sequencing